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KMID : 0861020140290040069
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Korea Journal of Herbology
2014 Volume.29 No. 4 p.69 ~ p.76
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Effect of Gami-Chunggisan on Antioxidant and Pro-Inflammatory Cytokine
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Lee Youn-Jeong
Sim Boo-Yong
Lee Hae-Jin
Bak Ji-Won
Kim Dong-Hee
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Abstract
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Objectives : Gami-Chunggisan extract (GCE) is one of the oriental traditional medicine. We investigated the antioxidant effect and reduction of pro-inflammatory cytokine as a functional ingredient for cosmetic products from the GCE.
Methods : GCE was prepared by extracting with 80% ethanol. We analyzed total polyphenol and antioxidant activities. To evaluate antioxidant activity, we measured 2,2``-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging assay. Also we measured the production of reactive oxygen species (ROS) and nitric oxide (NO) on Raw264.7 cells. We researched reduction of anti-inflammatory cytokines from concentration of GCE on Raw264.7 cells.
Results : Total polyphenol quantity of GCE was included 46.6 ㎎/g. The GCE showed ABTS free radical scavenging ability with more than 89% at 1000 ㎍/㎖. In addition the DPPH free radical scavenging ability from the GCE was activated over 93% at 1000 ㎍/㎖. Production of the ROS was decreased by approximately 26%, upon the GCE treatment at concentration of 100 ㎍/㎖. The GCE at 100 ㎍/㎖ concentration showed inhibitory effect on NO production by 38%. Production of IL-1β and IL-6 were decreased by approximately 56% and 36%, respectively upon GCE treatment at 100 ㎍/㎖. Also, production of TNF-α was decreased by approximately 79% at 100 ㎍/㎖. Moreover, the GCE showed inhibitory effects on the expression of the IL-1β, IL-6 and TNF-α genes in LPS-induced Raw 264.7 cells.
Conclusions : From the results above, we conclude that the GCE indicated significant antioxidant effects and induced reduction of pro-inflammatory cytokine.
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KEYWORD
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Gami-Chunggisan, antioxidant, free radical, anti-inflammation, cytokine
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